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Superoxide creation is actually detected within the bloodstream and you will BALF utilizing the CMH twist probe. Blood or BALF products have been obtained of PBS- and you will Bleo-addressed rats and you can incubated instantly with CMH. The fresh products were moved to the fresh PTFE tube and you can thumb suspended, and EPR analysis try gathered from the 77 K. The fresh intensity of nitroxide (CM.) gathered in the bloodstream incubated which have CMH (0.dos mM) in the 37 degrees to have ten minute (Shape 4A).

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